The SERVA IEF sample buffer is suited to all vertical and horizontal IEF applications and systems. The SERVA IEF sample buffer is supplied as 2x concentrate. It is sterile filtered, beneficial to long shelf life and absence of contaminants. The 2x concentrate is suited to liquid and solid samples.Simply mix the liquid sample 1:1 with the buffer or dissolve a solid sample in the buffer first and dilute with water 1:1. When performing IEF in the presence of urea mix the sample with the buffer and add solid urea or use concentrated urea solution.The buffer contains 4 % SERVALYT™ 4 - 9 T, 30 % glycerol and 0.005 % phenol red.